Neurodata Without Borders (NWB)¶

NWB is a data standard

Some movies are larger than the available RAM on users analysis computer. Below are several ways that the underlying functions in CIAPKG can be used to analyze large movies.

https://neurodatawithoutborders.github.io/matnwb/tutorials/html/ophys.html

Saving NWB¶

% Full path to the movie
saveNeurodataWithoutBorders(cellExtractionImages,{cellExtractionSignals},cellExtractionAlgorithm,nwbFilePath);

Where cellExtractionAlgorithm is the algorithm used, consisting of:¶

Loading NWB¶

% Full path to the movie
[inputImages,inputTraces,infoStruct, algorithmStr] = loadNeurodataWithoutBorders(nwbFilePath);

Outputs mean: - inputImages - 3D or 4D matrix containing cells and their spatial information. - inputTraces - 2D matrix containing trace outputs. - infoStruct - contains information about the file, e.g. the 'description' property that can contain information about the algorithm. - algorithmStr - String of the algorithm name.

Using NWB with signalSorter¶

For manual sorting, users can directly input path to NWB file as below:

[outImages, outSignals, choices] = signalSorter('pcaica.nwb',[],'inputMovie',inputMoviePath);